![]() Two orange reference bands at 70 kDa and 25 kDa and one green reference band at 10 kDa highlight the blue-stained protein ladder. Caution :Īll products are for research use only. Not intended for human or animal diagnostic or therapeutic uses. The PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250 kDa) for use in protein electrophoresis (SDS-PAGE) and western blotting. Note: The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. Under suggested conditions, BLUltra Prestained Protein Ladder resolves 10 major bands in 4-12% Bis-Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane. Prestained Protein Ladder V (approximately 0.20.4 mg/ml of each protein in. Each protein can be stained with a different color for easy identification or labeled with affinity tags for western blot detection. 2.5 μl per well for general Western transferring. Western Blotting, Sizing of proteins on SDS-PAGE and Western blots. Recombinant protein ladders are engineered to produce tight bands, evenly spaced molecular weights, and other specific traits.3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.► Enhanced transfer, wash, and stripping durabilities. ► Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system. ► Better performance for high molecular weight proteins. The Prestained Protein Ladder is a three-color protein standard with 12 pre. ► Higher Added Value: Tri-colors and 10 bands with premium quality. Western Blotting All Proteomics Chromatography. TIANGEN Pre-stained Protein Marker is a tri-color protein molecular weight standard with twelve pre-stained recombinant. ► One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa. Guide for Molecular Weight Estimation (kDa) : Invitrogen iBright Prestained Protein Ladder provide direct visualization during electrophoresis and transfer of Western blots. The ladder is supplied in the gel loading buffer and is ready to use. The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. BOX 5199, Limerick, Pennsylvania, USA.The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. ![]() Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. All claims must be made within 30 days following the date of delivery. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. Im looking for a prestained protein marker to run alongside my samples on SDS-PAGE. prestained ladder helps to track gel-to-membrane transfer during western blot. protein I am trying to identify is quite large-about 300kda. All properties listed are typical characteristics and are not specifications. prestained protein ladder is that unstained ladders require additional staining. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Please contact a technical service representative for more information. This product is for research use only and is not intended for therapeutic or diagnostic applications.
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